THE DETECTION OF THE VIRUSES IN CULTURED PENAEID SHRIBY DOUBLE ANTIBODY SANDWICH ELISA
双抗体夹心ELISA法检测养殖对虾病毒的研究

The anhsera against P. penicillatus spheroid--virus(PPSV) and baculovilal nddgut glandnecrosis virus (BMN'V) have been obtained from rabbitS and face by imlnedzillg the aamalsrespechvely with Purified viruses and a double anhbody sandwich ELISA procedure has beenestablihed to detect PPSV and BMNV from culled shrimp and from beachy aamals sUrroudingthe culal farms. The resultS showed that the double anhbody sandwich ELISA was high sensihveand 50ng Protein of PPSV and 100ng protein of BMN'V could be detected from 100UI ofhepatopancreatic homogenate. Vilal detechon in the cultUred P, penicillatUs and P. japocicus andbeachy kilnals indicated that a high rate of the cultUred shrimp and beachy aamals with normalappeaxance Proedy have been infected by viruses. The high specifity and reliability of the detechonwere confirmed by the observahons of TEM.