%0 Journal Article
%T THE DETECTION OF THE VIRUSES IN CULTURED PENAEID SHRIBY DOUBLE ANTIBODY SANDWICH ELISA<br>双抗体夹心ELISA法检测养殖对虾病毒的研究
%A Song Siyang
%A Luo Wenxin
%A Shen Mingshan
%A Chen Jinan
%A Su Wenjin
%A <br>宋思扬
%A 罗文新
%A 沈明山
%A 陈晋安
%A 苏文金
%J 微生物学通报
%D 1998
%I 
%X The anhsera against P. penicillatus spheroid--virus(PPSV) and baculovilal nddgut glandnecrosis virus (BMN'V) have been obtained from rabbitS and face by imlnedzillg the aamalsrespechvely with Purified viruses and a double anhbody sandwich ELISA procedure has beenestablihed to detect PPSV and BMNV from culled shrimp and from beachy aamals sUrroudingthe culal farms. The resultS showed that the double anhbody sandwich ELISA was high sensihveand 50ng Protein of PPSV and 100ng protein of BMN'V could be detected from 100UI ofhepatopancreatic homogenate. Vilal detechon in the cultUred P, penicillatUs and P. japocicus andbeachy kilnals indicated that a high rate of the cultUred shrimp and beachy aamals with normalappeaxance Proedy have been infected by viruses. The high specifity and reliability of the detechonwere confirmed by the observahons of TEM.
%K amble anhbody sandwich ELISA
%K Penaeid virus
%K Detechon
%K Culled shrimp
%K Beashyaamals<br>双抗体夹心ELISA
%K 对虾病毒
%K 检测
%K 养殖对虾
%K 近海岸生物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=3299F8A63198E2B38F157BD4C2D6D9F0&yid=8CAA3A429E3EA654&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=9971A5E270697F23&eid=6209D9E8050195F5&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=3&reference_num=9