RESTRICTION PATTERNS AND NUCLEOTIDE SEQUENCE OF THE POLYHEDRIN GENE OF SPODOPTERA LITURA NUCLEAR POLYHEDROSIS VIRUS
SINPV基因组酶切图谱及多角体基因的序列分析

Digestion of Spodoptera litura nuclear polyhedrosis virus (SINPV Guangzhou isolate) with restrictionendonucleases EcoRI.XbaI.Xhol.Bam HI Pst I SacI .Hind and SmaI resulted in 26.26.24.20. 13. 17.9 and 1fragments, respectively. The average size of the genome is about 136.OKbp. The polyhedrin gene of SINPV waslocated on the Xba I O fragments by southem hybridization methed using a DIG labeled AcMNPV polyhedringene as probe. With a coding sequence of 780 nucleotides, corresponding to a protein of 249 amino acid, thepolyhedrin gene of SINPV is the longest polyhedrin gene known to date together with the Spodoptera littoralisNPV polyhedrin gene. This gene codes for a putative protein with an Mr 29236 and the predicted amino acidsequence shows high homology with those of other NPVs.