%0 Journal Article
%T RESTRICTION PATTERNS AND NUCLEOTIDE SEQUENCE OF THE POLYHEDRIN GENE OF SPODOPTERA LITURA NUCLEAR POLYHEDROSIS VIRUS<br>SINPV基因组酶切图谱及多角体基因的序列分析
%A Wei Yongjie
%A Long Qixin
%A Chen Shangwu
%A Wang Xunzhang
%A <br>魏永杰
%A 龙綮新
%A 陈尚武
%A 王珣章
%J 微生物学通报
%D 1999
%I 
%X Digestion of Spodoptera litura nuclear polyhedrosis virus (SINPV Guangzhou isolate) with restrictionendonucleases EcoRI.XbaI.Xhol.Bam HI Pst I SacI .Hind and SmaI resulted in 26.26.24.20. 13. 17.9 and 1fragments, respectively. The average size of the genome is about 136.OKbp. The polyhedrin gene of SINPV waslocated on the Xba I O fragments by southem hybridization methed using a DIG labeled AcMNPV polyhedringene as probe. With a coding sequence of 780 nucleotides, corresponding to a protein of 249 amino acid, thepolyhedrin gene of SINPV is the longest polyhedrin gene known to date together with the Spodoptera littoralisNPV polyhedrin gene. This gene codes for a putative protein with an Mr 29236 and the predicted amino acidsequence shows high homology with those of other NPVs.
%K Spodoptera litura Nuclear Polyhedrosis Virus
%K Restriction endonuclease patterns
%K Polyhedrin gene The nucleotide sequence of polyhedrin gene of SINPV has been submitted to the GeneBank/EMBL Data
%K libraries
%K under the accession number AF 037262<br>斜纹夜蛾核型多角体病毒
%K 限制性内切酶图谱
%K 多角体基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=0B100A5EC19CFD1E63B54AEE7A573C48&yid=B914830F5B1D1078&vid=96C778EE049EE47D&iid=0B39A22176CE99FB&sid=7E8E8B150580E4AB&eid=08805F9252973BA4&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=6&reference_num=1