SELECTION HIGH-YIELD RIBOFLAVIN PRODUCING STRAIN OF PROTOPLASTS TREATMENTED WITH UV
紫外线诱变原生质体选育核黄素高产菌株

In this paper, factors affecting protoplast formation, the microscopic observation of the process of protopplast formation and regeneration, the distribution of Riboflavins productivity among the protoplast regenerated clonies treated with UV were investigated.Protoplasts were obtained from mycelia of Eremothecium ashbyii by using a combined lyric enzyme system containing commercial cellulase and snail digestase. The frequence of the protoplast regeneration was 6.1&, The high-yield stable strain UP-91 was obtained by UV irradiation of protoplast and screening of the fermentation of many regenerative mutants. The productivity of the mutant was as 30% high as that of starting strain.