%0 Journal Article
%T SELECTION HIGH-YIELD RIBOFLAVIN PRODUCING STRAIN OF PROTOPLASTS TREATMENTED WITH UV<br>紫外线诱变原生质体选育核黄素高产菌株
%A Cheng Lurong Deng Xiuzhen Jiang Youmin Ge Zhonglian
%A <br>程鲁榕
%A 邓秀珍
%A 江幼岷
%A 葛忠良
%J 微生物学通报
%D 1993
%I 
%X In this paper, factors affecting protoplast formation, the microscopic observation of the process of protopplast formation and regeneration, the distribution of Riboflavins productivity among the protoplast regenerated clonies treated with UV were investigated.Protoplasts were obtained from mycelia of Eremothecium ashbyii by using a combined lyric enzyme system containing commercial cellulase and snail digestase. The frequence of the protoplast regeneration was 6.1&, The high-yield stable strain UP-91 was obtained by UV irradiation of protoplast and screening of the fermentation of many regenerative mutants. The productivity of the mutant was as 30% high as that of starting strain.
%K Eremothecium ashbyii
%K Riboflavin-producing
%K Protoplast
%K Mutatioa breeding<br>阿舒假囊酵母菌
%K 原生质体
%K 诱变育种
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=3FF2D3B4679822EBF18C53650180E040&yid=D418FDC97F7C2EBA&vid=A04140E723CB732E&iid=CA4FD0336C81A37A&sid=2A8D03AD8076A2E3&eid=BC12EA701C895178&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=3&reference_num=0