Simultaneous Determination of Seven Ginsenoside Derivatives in Dog Plasma by LC-MS/MS: Application to Pharmacokinetic Study of Ginseng Extraction
LC-MS/MS法同时测定犬血浆中7 种人参皂苷类成分及其在人参提取物药代动力学研究中的应用

A sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) was developed and validated for the simultaneous quantification of Ginsenoside Rg 1,Re,Rb 1,Rd,Rc,Rb 2/3 and Rf in dog plasma.The compounds were extracted by organic solvent using mixed organic solvent.Chromatographic separation was achieved on a reversed-phase C 18 column with the mobile phase of acetonitrile/menthol-water(0.01% acetic acid) and step gradient elution resulted at a flow rate of 0.23 mL.min-1.A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode.Quantification was performed using multiple reaction monitoring(MRM) of the transitions m/z 1131.8/789.5(G-Rb 1),1101.7/789.7(G-Rb 2/3),1101.7/789.7(G-Rc),823.6/789.5(G-Rd),969.7/789.7(G-Re),823.6/643.6(G-Rg 1) and 823.6/ 365.3(G-Rf).Seven ginsenosides were eluted within 14.5 min and can avoid matrix effect.The quantification limits of the newly developed method were 0.03125 μg.L-1 for G-Re and G-Rg 1,0.0625 μg.L-1 for G-Rf and G-Rd,0.125 μg.L-1 for G-Rb 1,G-Rc and G-Rb 2/3.The precision,accuracy and stability of the analytes meet the requirements.The method was sensitive,accurate and rapid.It can be applied in the determination of plasma concentration of seven ginsenosides.