%0 Journal Article
%T Simultaneous Determination of Seven Ginsenoside Derivatives in Dog Plasma by LC-MS/MS: Application to Pharmacokinetic Study of Ginseng Extraction<br>LC-MS/MS法同时测定犬血浆中7 种人参皂苷类成分及其在人参提取物药代动力学研究中的应用
%A Lin Li
%A Liu Jianxun
%A Zhang Ying
%A Li Xinzhi
%A Duan Cangling
%A Lin Chengren
%A Li Lei
%A <br>林力
%A 刘建勋
%A 张颖
%A 李欣志
%A 段昌令
%A 林成仁
%A 李磊
%J 世界科学技术-中医药现代化
%D 2012
%I 
%X A sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) was developed and validated for the simultaneous quantification of Ginsenoside Rg 1,Re,Rb 1,Rd,Rc,Rb 2/3 and Rf in dog plasma.The compounds were extracted by organic solvent using mixed organic solvent.Chromatographic separation was achieved on a reversed-phase C 18 column with the mobile phase of acetonitrile/menthol-water(0.01% acetic acid) and step gradient elution resulted at a flow rate of 0.23 mL.min-1.A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode.Quantification was performed using multiple reaction monitoring(MRM) of the transitions m/z 1131.8/789.5(G-Rb 1),1101.7/789.7(G-Rb 2/3),1101.7/789.7(G-Rc),823.6/789.5(G-Rd),969.7/789.7(G-Re),823.6/643.6(G-Rg 1) and 823.6/ 365.3(G-Rf).Seven ginsenosides were eluted within 14.5 min and can avoid matrix effect.The quantification limits of the newly developed method were 0.03125 μg.L-1 for G-Re and G-Rg 1,0.0625 μg.L-1 for G-Rf and G-Rd,0.125 μg.L-1 for G-Rb 1,G-Rc and G-Rb 2/3.The precision,accuracy and stability of the analytes meet the requirements.The method was sensitive,accurate and rapid.It can be applied in the determination of plasma concentration of seven ginsenosides.
%K Ginsenoside
%K pharmacokinetics
%K LC-MS/MS<br>人参皂苷
%K 药代动力学
%K 液质联用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=B5F35A1FC27C1FF7&jid=44257A35316DB0727BA6BC707B9EDAAB&aid=9B3C227A81E2A28C7F32A46CC8F4EE23&yid=99E9153A83D4CB11&vid=F3583C8E78166B9E&iid=38B194292C032A66&sid=FAE014814EB8ABBA&eid=209749DE0D716184&journal_id=1674-3849&journal_name=世界科学技术-中医药现代化&referenced_num=0&reference_num=0