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OALib Journal期刊
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Measurement & Analysis of Sargassum Fusiforme Polysaccharides
羊栖菜多糖含量测定及多糖组分分析

Keywords: 羊栖菜,多糖,含量测定,硫酸苯酚法,高效毛细管电泳

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Abstract:

This study is made to measure the concentration of polysaccharide in Sargassum fusiforme, and analyze the monosaccharide composition derived, in an effort to provide some helpful insights for future researches in chemistry and pharmacology. A UV-spectrophotometer and phenol -sulfuric method are used, with a detection wavelength at 490nm. The monosaccharide composition is determined using HPCE. The findings show that Sargassum fusiforme may have a polysaccharide concentration as high as 53.46%, with an average recovering rate of 99.37%, or RSD=2.19%. Polysaccharides derived from Sargassum fusiforme is made up of Rhamnose, Xylose, Glucose, Mannose, Fructose, Fucoidan, Galactose and a unknown element. Authors believe that the phenol-sulfuric acid method has proved simple, convenient and reliable, and HPCE can be used to analyze the composition of polysaccharides.

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