Comparative Studies on Determination of Polysacharide in Dendrobium officinale
“永生源”铁皮石斛中多糖含量测定方法的比较研究

This work aimed to select a better assay method for polysacharide in Dendrobium officinale. Phenol-sulfuric acid colorimetry and 3,5-dinitrosalicylic acid colorimetry (DNS) were respectively adopted to determine watersoluble polysaccharide. The content of polysaccharide determined by phenol-sulfuric acid colorimetry was higher than by 3, 5-dinitrosalicylic acid colorimetry. The results showed that 3, 5-dinitrosalicylic acid colorimetry is more suitable for the determination of polysacharide in D. officinale. This method has been proven to be simple, stable and repeatable, thus may be used for the quality control of crude D. officinale and medical care preparations containing polysaccharide extracted from D. officinale.