%0 Journal Article
%T Comparative Studies on Determination of Polysacharide in Dendrobium officinale<br>“永生源”铁皮石斛中多糖含量测定方法的比较研究
%A Liu Hongyuan
%A Yang Jinyan
%A Huang Song
%A Huang Bin
%A Lai Xiaopin
%A <br>刘宏源
%A 杨金燕
%A 黄 松
%A 黄 斌
%A 赖小平
%J 世界科学技术-中医药现代化
%D 2010
%I 
%X This work aimed to select a better assay method for polysacharide in Dendrobium officinale. Phenol-sulfuric acid colorimetry and 3,5-dinitrosalicylic acid colorimetry (DNS) were respectively adopted to determine watersoluble polysaccharide. The content of polysaccharide determined by phenol-sulfuric acid colorimetry was higher than by 3, 5-dinitrosalicylic acid colorimetry. The results showed that 3, 5-dinitrosalicylic acid colorimetry is more suitable for the determination of polysacharide in D. officinale. This method has been proven to be simple, stable and repeatable, thus may be used for the quality control of crude D. officinale and medical care preparations containing polysaccharide extracted from D. officinale.
%K Dendrobium officinale
%K Polysaccharide
%K Phenol-sulfuric acid colorimetry
%K 3
%K 5-Dinitrosalicylic acid colorimetry
%K Content determination<br>铁皮石斛
%K 多糖
%K 苯酚-浓硫酸比色法
%K 3，5-二硝基水杨酸比色法
%K 含量测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=B5F35A1FC27C1FF7&jid=44257A35316DB0727BA6BC707B9EDAAB&aid=656E32E02735604BAC3B5F6E70CBC82C&yid=140ECF96957D60B2&vid=59906B3B2830C2C5&iid=94C357A881DFC066&sid=B3645A659773B73C&eid=0018E43E61963A72&journal_id=1674-3849&journal_name=世界科学技术-中医药现代化&referenced_num=0&reference_num=0