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Cloning of Critical Gene Fragments in Saikosaponin Biosynthesis and Associated Sequence Analysis
柴胡皂苷合成途径中三个关键酶基因片段的克隆与序列分析

Keywords: Bupleurum chinense DC,saikosaponin,3-hydroxy-3-methylglutaryl coenzyme A reductase,isopentenyl diphosphate isomerase,farnesyl diphosphate synthase
北柴胡,柴胡皂苷,3-羟基-3甲基戊二酰辅酶A还原酶(HMGR),异戊烯基焦磷酸异构酶(IPPI),法尼基焦磷酸合酶(FPS)

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Abstract:

cDNA fragments,encoding HMGR,IPPI and FPS in Bupleurum chinense DC.were amplified by RT-PCR with the total RNA of the young roots as a template,cDNA fragments of HMGR (GenBank accession no.EU400217),IPPI (EU400218),and FPS (EU400219) were 470bp,532bp,466bp in length,encoding 157,177,155 amino acids respectively.The deduced amino acid sequence of cDNA encoding HMGR,IPPI and FPS showed the highest amino acid identity to E.ulmoides (93%),B.falcatum (99%),and C.asiatica (97%) respectively.The deduced amino acid...

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