Study on the Mechanisms of NRK Cells Apoptosis Induced by Cadmium
镉诱导大鼠肾细胞凋亡及其机理的研究

In order to study the death-receptor pathway in NRK cell apoptosis induced by cadmium, different concentrations of cadmium chloride (0, 10, 20, 40, 60mmol·L-1) were added to NRK cell culture medium and cultured for 6h. Apoptosis rate, Caspase-8 activity and expression level of mRNA of Caspase-8 and Fas were detected by flow cytometry, spectrophotometer and RT-PCR, respectively. Results showed that concomitant dose-dependent increases in apoptosis rate, Caspase-8 activity and the expression level of Caspase-8 and Fas mRNA were found, which indicated apoptosis of NRK cells could be induced by cadmium through death-receptor pathway.