Cloning and Sequence Analysis of Trachinotus ovatus Mitochondrial 16S rRNA Gene
卵形鲳!线粒体１６ＳｒＲＮＡ基因全长序列的克隆与分析

Based on multiple alignment of mitochondrial sequence from neighbored spices, a pair of conserved primers was designed on the flanking region of 16S rRNA gene. The PCR amplification product was cloned to PGEM-Teasy vector and sequenced. Blast on NCBI confirmed that the sequence contain whole 1725bp of 16S rRNA gene. Alignment of sequence from 5 fishes of the same population revealed 7 variation sites, which form 5 different genotypes. Based on the multiple alignment of 16S rRNA sequence of Trachinotus ovatus and other Carangidae species, a phylogenetic tree was constructed which supporting the idea that Carangidae could be divided into four subfamilies: Caranginae, Seriolinae, Trachinotinae and Chorineminae. So, 16S rRNA gene is a useful tool which can be used not only in genetic polymorphism analysis of Trachinotus ovatus, but also in phylogenetic analysis of Caranginae species.