%0 Journal Article
%T Cloning and Sequence Analysis of Trachinotus ovatus Mitochondrial 16S rRNA Gene<br>卵形鲳!线粒体１６ＳｒＲＮＡ基因全长序列的克隆与分析
%A PENG Jin-xia
%A PENG Min
%A CHEN Xiu-li
%A JIANG Wei-ming
%A YANG Chun-ling
%A LI Yong-mei
%A <br>彭金霞
%A 彭敏
%A 陈秀荔
%A 蒋伟明
%A 杨春玲
%A 李咏梅
%J 水生态学杂志
%D 2010
%I 
%X Based on multiple alignment of mitochondrial sequence from neighbored spices, a pair of conserved primers was designed on the flanking region of 16S rRNA gene. The PCR amplification product was cloned to PGEM-Teasy vector and sequenced. Blast on NCBI confirmed that the sequence contain whole 1725bp of 16S rRNA gene. Alignment of sequence from 5 fishes of the same population revealed 7 variation sites, which form 5 different genotypes. Based on the multiple alignment of 16S rRNA sequence of Trachinotus ovatus and other Carangidae species, a phylogenetic tree was constructed which supporting the idea that Carangidae could be divided into four subfamilies: Caranginae, Seriolinae, Trachinotinae and Chorineminae. So, 16S rRNA gene is a useful tool which can be used not only in genetic polymorphism analysis of Trachinotus ovatus, but also in phylogenetic analysis of Caranginae species.
%K Trachinotus ovatus
%K mitochondrial
%K 16S rRNA gene
%K genetic resource
%K phylogenetic tree<br>卵形鲳鲹
%K 线粒体
%K 16S
%K rRNA基因
%K 种质资源
%K 系统发育进化树
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=A129AB11A5573C59&jid=AD42C12863085B5F664F8056EF6D57C2&aid=9F97AC108B1C340C31C02A560A8B44FB&yid=140ECF96957D60B2&vid=4AD960B5AD2D111A&iid=E158A972A605785F&sid=35FC3610259C2B32&eid=CD775AE9DDBD7B53&journal_id=1674-3075&journal_name=水生态学杂志&referenced_num=0&reference_num=0