The Establishment of a Gastric Cancer Cell Line Expressed Enhanced Green Fluorescent Protein (EGFP) Induced by Dioxin-like Chemicals
一株受二噁英类化合物诱导表达绿色荧光蛋白的胃癌细胞系的建立

Five joined XRE fragments and minimal CMV (Human cytomegalovirus) promoter were inserted into pEGFP-1 to generate a new vector pXRE5-EGFP. When human gastric cancer cell line SGC-7901 was stably transfected with this construct, the recombinant cell line XRE5-SGC-7901 expressed enhanced green fluorescent protein (EGFP) following stimulation with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). After exposed to different concentrations of TCDD (0, 250, 500pg·mL-1), the cell line expressed EGFP in dose-dependent manners. These results suggested that XRE5-SGC-7901 was a useful bioassay system for dioxin-like chemicals.