%0 Journal Article
%T The Establishment of a Gastric Cancer Cell Line Expressed Enhanced Green Fluorescent Protein (EGFP) Induced by Dioxin-like Chemicals<br>一株受二噁英类化合物诱导表达绿色荧光蛋白的胃癌细胞系的建立
%A WANG Chang
%A WANG Ying
%A LEI Yao
%A ZHANG Jie
%A ZHENG Ming-hui
%A LIANG Yong
%A <br>汪畅
%A 王英
%A 雷垚
%A 张捷
%A 郑明辉
%A 梁勇
%J 生态毒理学报
%D 2008
%I 
%X Five joined XRE fragments and minimal CMV (Human cytomegalovirus) promoter were inserted into pEGFP-1 to generate a new vector pXRE5-EGFP. When human gastric cancer cell line SGC-7901 was stably transfected with this construct, the recombinant cell line XRE5-SGC-7901 expressed enhanced green fluorescent protein (EGFP) following stimulation with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). After exposed to different concentrations of TCDD (0, 250, 500pg·mL-1), the cell line expressed EGFP in dose-dependent manners. These results suggested that XRE5-SGC-7901 was a useful bioassay system for dioxin-like chemicals.
%K dioxin-like chemicals
%K induced expression vector
%K green fluorescent protein<br>二噁英类化合物
%K 诱导表达载体
%K 绿色荧光蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=BEC1F4700DD66C14D137590A0896A0AD&jid=B98D390DF92C35E6DD1987675E4EE86E&aid=C442BE0ED7F944BFCEA5405FF9A2C2E2&yid=67289AFF6305E306&vid=38B194292C032A66&iid=38B194292C032A66&sid=5B5B75F4854B8331&eid=D537C66B6404FE57&journal_id=1673-5897&journal_name=生态毒理学报&referenced_num=0&reference_num=20