RESEARCH ON INCREASE OF MEMBRANE FLUIDTY OF PLANTMITOCHONDRIA BY ABSCISIC ACID WITH ANS
用ANS研究脱落酸提高植物线粒体膜的流动性

The effect of abscisic acid (ABA) on the membrane fluidity of maize mitochondria was studied by the method of fluorescence labeling of 8-anilino-1-naphthalene-sulfonic acid (ANS). The results from the experiments of ( )ABA concentrations, pH and temperature showed that ( )ABA could all decrease the fluorescence intensity, namely, ( )ABA could enhance the membrane fluidity of ANS incubating with plant mitochondria. The saturation phenomenon was appeared in the effect of ( )ABA. Which resulted in the increase of isocitrate dehydrogenase (ICDH) activity. The analogs of ABA, RCA.7a and RCA.7b, could also increase the ICDH activity. However, polyclonal antibodies against ABA-binding protein (anti-ABBP PAbs) could inhibit such effect of ( )ABA. All of the above results suggested the existence the ABA receptor site in the mitochondria membrane.