%0 Journal Article %T RESEARCH ON INCREASE OF MEMBRANE FLUIDTY OF PLANTMITOCHONDRIA BY ABSCISIC ACID WITH ANS
用ANS研究脱落酸提高植物线粒体膜的流动性 %A CHEN Kao-shan %A LIU Shi-ming %A XIA Kai %A ZHOU Xie %A
陈靠山 %A 刘世名 %A 夏凯 %A 周燮 %J 生物物理学报 %D 2000 %I %X The effect of abscisic acid (ABA) on the membrane fluidity of maize mitochondria was studied by the method of fluorescence labeling of 8-anilino-1-naphthalene-sulfonic acid (ANS). The results from the experiments of ( )ABA concentrations, pH and temperature showed that ( )ABA could all decrease the fluorescence intensity, namely, ( )ABA could enhance the membrane fluidity of ANS incubating with plant mitochondria. The saturation phenomenon was appeared in the effect of ( )ABA. Which resulted in the increase of isocitrate dehydrogenase (ICDH) activity. The analogs of ABA, RCA.7a and RCA.7b, could also increase the ICDH activity. However, polyclonal antibodies against ABA-binding protein (anti-ABBP PAbs) could inhibit such effect of ( )ABA. All of the above results suggested the existence the ABA receptor site in the mitochondria membrane. %K ABA %K Mitochondria %K Membrane fluidity
ABA %K 线粒体 %K 脱流动性 %K 荧光标记法 %K 受体位点 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=DFC6750FD6A19356&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=0B39A22176CE99FB&sid=FE4C96E058BB2280&eid=0C191C6ECF79047F&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=2&reference_num=5