Potassium Currents in Neurons of Rats Were Reduced by IL-1β in Time Dependent Manner
白介素1β时间依赖性地抑制大鼠皮层神经元钾电流

Interleukin-1β (IL-1β) is an important porinflammatory cytokine that exert widely biology functions in central nervous system (CNS). Many Studies had reported that the effects of IL-1β were complicated. These effects include both of neuroprotective and neurotoxic effects in different models or conditions. Voltage-gated K+ channels contribute to the excitability of neurons, and regulate shape of action potential. Although both of IL-1β and K+ channels play key roles in injury and disease of brain, so far the relations of them was reported in few studies. In this study, whole cell patch-clamp recording was used to investigate the effects of IL-1β (10 ng/mL) treatment for 8 and 24 hours on voltage-dependent K+ currents of cultured cortical neurons from rats. Base on the properties of the currents, K+ currents were divided into two components, i.e. transient outward potassium current (IA)-dominant IA-like and delayed rectifier potassium current (IK)-dominant IK-like currents. The results showed that both of the K+ currents in cortical neurons were not affected by IL-1β treatment for 8 hours, but significantly inhibited by treatment for 24 hours. Our results suggested that the voltage-gated K+ currents were inhibited by IL-1β treatment in time dependent manner in cortical neurons of rats.