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水生态学杂志 2011
Isolation and Characterization of Microsatellite Loci in Spinibarbus sinensis(Bleeker)
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Abstract:
The microsatellite markers of Spinibarbus sinensis were isolated in this study. A library of partial small size fractionated genomic DNA was constructed with the S. sinensis in Wujiang river. Eight microsatellite loci were isolated and characterized. These loci showed high levels of genetic polymorphism testing on 32 individuals sampled from Wujiang river. The number of alleles per locus ranged from 4 to 10. PIC changed from 0.6072 to 0.8515. The observed (Ho) and expected (He) heterozygosities were 0.5625 to 1.0000 and 0.6791 to 0.8795, respectively. Hardy-Weinberg departure value (D) ranged from -0.2009 to 0.3805. The Hardy-Weinberg Equilibrium (HWE) analysis showed that 3 loci deviated from genetic equilibrium significantly (P<0.05). No linkage disequilibrium was found.
