%0 Journal Article
%T Isolation and Characterization of Microsatellite Loci in Spinibarbus sinensis(Bleeker)<br>中华倒刺鲃微卫星标记筛选及特征分析
%A xiong meim hu
%A shi fang
%A xu lian
%A que yan fu
%A yang zhong
%A hu ju xiang
%A zhu bing
%A <br>熊美华
%A 史　方
%A 徐　念
%A 阙延福
%A 杨　钟
%A 胡菊香
%A 朱　滨
%J 水生态学杂志
%D 2011
%I 
%X The microsatellite markers of Spinibarbus sinensis were isolated in this study. A library of partial small size fractionated genomic DNA was constructed with the S. sinensis in Wujiang river. Eight microsatellite loci were isolated and characterized. These loci showed high levels of genetic polymorphism testing on 32 individuals sampled from Wujiang river. The number of alleles per locus ranged from 4 to 10. PIC changed from 0.6072 to 0.8515. The observed (Ho) and expected (He) heterozygosities were 0.5625 to 1.0000 and 0.6791 to 0.8795, respectively. Hardy-Weinberg departure value (D) ranged from -0.2009 to 0.3805. The Hardy-Weinberg Equilibrium (HWE) analysis showed that 3 loci deviated from genetic equilibrium significantly (P<0.05). No linkage disequilibrium was found.
%K Spinibarbus sinensis
%K enriched genomic library
%K microsatellite
%K polymorphism<br>中华倒刺鲃
%K 基因组富集文库
%K 微卫星
%K 多态性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=A129AB11A5573C59&jid=AD42C12863085B5F664F8056EF6D57C2&aid=95C4B21264D443C02078E3B4D53FF8E4&yid=9377ED8094509821&vid=9971A5E270697F23&iid=CA4FD0336C81A37A&sid=1371F55DA51B6E64&eid=1AE5323881A5ECDC&journal_id=1674-3075&journal_name=水生态学杂志&referenced_num=0&reference_num=0