Influence of fusion protein of IBDV VP2 and chicken Interleukin-2 on immune response in chicken
禽IL-2与传染性法氏囊VP2融合蛋白免疫学特性

In order to research immunogenicity of the recombinant rVP2-IL-2 fusion protein,we obtained the rVP2-IL-2 fusion protein using Pichia pastoris expression system,and then evaluated its potential to induce immune responses in chicken.The effect was determined in the form of protective anti-IBDV VP2 titers,antibodies(IgG1 and IgG2a),lymphocyte proliferation,the levels of interferon-7 and interleukin-4 cytokines,and challenge experiment.Antibody titers and proliferation lymphocyte level suggested that the fusion protein could elicit specific humoral immune and cellular immune responses.antibody sub-type results indicated that the rVP2-IL-2 fusion protein induced secretion both of IgGl and IgG2a.The seem result elicited from cytokines ELISA test,secretion of both ofThl(γ-IFN)and Th2(IL-4)were induced by the rVP2-IL-2 fusion protein.Challenge experiment result shown that chicken immunized the rVP2-IL-2 fusion protein obtained 85%protection.These results confirm that the fusion protein enhances the protection against IBDV through both humoral and cell-mediated immunity,and thus could Sgl"Ve as a candidate for the development of IBDV subunit vaccine.