%0 Journal Article
%T Influence of fusion protein of IBDV VP2 and chicken Interleukin-2 on immune response in chicken<br>禽IL-2与传染性法氏囊VP2融合蛋白免疫学特性
%A Chen Wang
%A Zhanqin Zhao
%A Chunjie Zhang
%A Yichen Liu
%A Ke Ding
%A Yinju Li
%A Xiangchao Cheng
%A Puyan Chen
%A <br>王臣
%A 赵战勤
%A 张春杰
%A 刘一尘
%A 丁轲
%A 李银聚
%A 程相朝
%A 陈溥言
%J 生物工程学报
%D 2010
%I 
%X In order to research immunogenicity of the recombinant rVP2-IL-2 fusion protein,we obtained the rVP2-IL-2 fusion protein using Pichia pastoris expression system,and then evaluated its potential to induce immune responses in chicken.The effect was determined in the form of protective anti-IBDV VP2 titers,antibodies(IgG1 and IgG2a),lymphocyte proliferation,the levels of interferon-7 and interleukin-4 cytokines,and challenge experiment.Antibody titers and proliferation lymphocyte level suggested that the fusion protein could elicit specific humoral immune and cellular immune responses.antibody sub-type results indicated that the rVP2-IL-2 fusion protein induced secretion both of IgGl and IgG2a.The seem result elicited from cytokines ELISA test,secretion of both ofThl(γ-IFN)and Th2(IL-4)were induced by the rVP2-IL-2 fusion protein.Challenge experiment result shown that chicken immunized the rVP2-IL-2 fusion protein obtained 85%protection.These results confirm that the fusion protein enhances the protection against IBDV through both humoral and cell-mediated immunity,and thus could Sgl"Ve as a candidate for the development of IBDV subunit vaccine.
%K infectious bursal disease virus (IBDV)
%K VP2 gene
%K IL-2
%K fusion protein<br>传染性法氏囊，VP2基因，IL-2，融合蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=233114C2904ED394A19E5CBCC191BBEB&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=DA4893B5F9885621&eid=BE5DBC360CD4FFB9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=4