High expression of antimicrobial peptide Cecropin AD in Escherichia coli by fussion with EDDIE
利用融合蛋白EDDIE在大肠杆菌中高效表达抗菌肽Cecropin AD

In this study,we efficiently expressed the active antimicrobial peptide(CAD),which fused with the site-mutated coat protein(EDDIE) of the classical swine fever virus,in Escherichia coli.First,we obtained the e-cad fusion gene from the CAD gene and the EDDIE gene using overlapping PCR.Then to get the recombinant expression vector(pETED),the e-cad fusion gene was cloned into the pET30a vector by a site-directed homologous recombination technique.The EDDIE-CAD fusion protein expressed in E.coli as inclusion bo...