%0 Journal Article
%T High expression of antimicrobial peptide Cecropin AD in Escherichia coli by fussion with EDDIE<br>利用融合蛋白EDDIE在大肠杆菌中高效表达抗菌肽Cecropin AD
%A Zhen Zhang
%A Tao Ke
%A Yuling Zhou
%A Xiangdong Ma
%A Lixin Ma
%A <br>张贞
%A 柯涛
%A 周玉玲
%A 马向东
%A 马立新
%J 生物工程学报
%D 2009
%I 
%X In this study,we efficiently expressed the active antimicrobial peptide(CAD),which fused with the site-mutated coat protein(EDDIE) of the classical swine fever virus,in Escherichia coli.First,we obtained the e-cad fusion gene from the CAD gene and the EDDIE gene using overlapping PCR.Then to get the recombinant expression vector(pETED),the e-cad fusion gene was cloned into the pET30a vector by a site-directed homologous recombination technique.The EDDIE-CAD fusion protein expressed in E.coli as inclusion bo...
%K antimicrobial peptides
%K fusion protein
%K high expression<br>抗菌肽
%K 融合蛋白
%K 高效表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C62F15B0203D529D1EFEA28BEB3A8E7D&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=5D311CA918CA9A03&sid=8C8B0901DFA6ECA2&eid=9E9FFC2CDB44A291&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=16