Expression, purification and immunogenicity analysis of HPV type 18 virus-like particles from Escherichia coli
人乳头瘤病毒18型病毒样颗粒在大肠杆菌中的表达及免疫原性分析

Here, we presented a method to bacterially express the major structural protein L1 of Human Papillomavirus type 18 (HPV18) as soluble form. We found that the purified L1 could self-assemble to virus-like particles (VLPs). Further, we investigated the immunogenicity and the induced level of neutralizing antibody using these VLPs. First, the genome of HPV18 was cloned from a patient in Xiamen. It was used as template for PCR amplification of HPV18 L1 gene. The resultant DNA fragment was inserted into expressi...