%0 Journal Article
%T Expression, purification and immunogenicity analysis of HPV type 18 virus-like particles from Escherichia coli<br>人乳头瘤病毒18型病毒样颗粒在大肠杆菌中的表达及免疫原性分析
%A Minghui Xie
%A Shaowei Li
%A Wentong Shen
%A Zhongyi Li
%A Yudi Zhuang
%A Xiaobing Mo
%A Ying Gu
%A Ting Wu
%A Jun Zhang
%A Ningshao Xia
%A <br>谢明辉
%A 李少伟
%A 沈文通
%A 李仲艺
%A 庄玉娣
%A 莫小兵
%A 顾颖
%A 吴婷
%A 张军
%A 夏宁邵
%J 生物工程学报
%D 2009
%I 
%X Here, we presented a method to bacterially express the major structural protein L1 of Human Papillomavirus type 18 (HPV18) as soluble form. We found that the purified L1 could self-assemble to virus-like particles (VLPs). Further, we investigated the immunogenicity and the induced level of neutralizing antibody using these VLPs. First, the genome of HPV18 was cloned from a patient in Xiamen. It was used as template for PCR amplification of HPV18 L1 gene. The resultant DNA fragment was inserted into expressi...
%K Escherichia coli
%K human papillomavirus type 18
%K virus-like particles
%K immunogenicity
%K neutralizing antibody<br>大肠杆菌表达系统
%K 人乳头瘤病毒18型
%K 病毒样颗粒
%K 免疫原性
%K 中和抗体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=042F96576A7FEC47F18AAD7CC2162067&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=DF92D298D3FF1E6E&sid=FA63B973BAB5E93D&eid=DE3C727FE2D9509D&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=11