Optimization of high-cell-density fermentation process for S-adenosyl-L-methionine production
S-腺苷-L-蛋氨酸高密度发酵工艺优化

Poor stability existed in the anaphase of the high-cell-density fermentation of Saccharomyces crevisiae for S-adenosyl-L-methionine (SAM) production in 5 L fermentor. To improve the fermentation stability,we studied the addition of diammonium hydrogen phosphate,sodium glutamate and adenosine disodium triphosphate into glucose feeding solution. Study of four fed-batch cultures showed that,after 34 h fermentation,when dry cell weight reached 100 g/L,the addition of 50 g pre-L-methionine and glucose feeding wi...