Construction of Saccharomyces cerevisiae mutant with knockout of SNF4 gene
酿酒酵母SNF4基因敲除缺失菌株的构建

Construction and ethanol production effects of SNF4 gene knockout in Saccharomyces cerevisiae were described in this paper.For knockout of SNF4 gene in S.cerevisiae YS2,a PCR-amplified disruption cassette was used,encoding the short flanking homologous regions to the SNF4 gene and Kanr as selectable marker.The SNF4 gene disruption cassette was transformed into S.cerevisiae YS2 through LiAc/SS Carrier DNA/PEG.The positive transformants were grown on G418 plates and verified by PCR.The Kanr marker was rescued...