%0 Journal Article
%T Construction of Saccharomyces cerevisiae mutant with knockout of SNF4 gene<br>酿酒酵母SNF4基因敲除缺失菌株的构建
%A Xiaohua Lin
%A Chongrong Ke
%A Bisha Wu
%A Yongbiao Zheng
%A Li Li
%A Youqiang Chen
%A Jianzhong Huang
%A <br>林晓华
%A 柯崇榕
%A 吴毕莎
%A 郑永标
%A 李力
%A 陈由强
%A 黄建忠
%J 生物工程学报
%D 2011
%I 
%X Construction and ethanol production effects of SNF4 gene knockout in Saccharomyces cerevisiae were described in this paper.For knockout of SNF4 gene in S.cerevisiae YS2,a PCR-amplified disruption cassette was used,encoding the short flanking homologous regions to the SNF4 gene and Kanr as selectable marker.The SNF4 gene disruption cassette was transformed into S.cerevisiae YS2 through LiAc/SS Carrier DNA/PEG.The positive transformants were grown on G418 plates and verified by PCR.The Kanr marker was rescued...
%K Saccharomyces cerevisiae
%K SNF4
%K gene knockout<br>酿酒酵母，SNF4，基因敲除
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B3D5074A6E7C001F1E10E553DB4001B1&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=E158A972A605785F&sid=6A9657F54F754BF6&eid=C2053D4E59904B8A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=22