Application of competitive PCR for screening selectable marker-free Xa21 transgenic rice
利用竞争性PCR筛选无标记Xa21转基因水稻

Polymerase chain reaction (PCR) is a simple,quick and highly sensitive method. However the accuracy of the conventional PCR assay was often affected by false positives and false negatives. In this study,a protocol competitive PCR was used to reduce the false results in screening for selectable marker-free (SMF) Xa21 transgenic rice plants. The competitive template of Xa21 was the endogenous Xa21 homologous sequence located on chromosome 11. The competitive template of the selectable marker gene,hygromycin p...