%0 Journal Article
%T Application of competitive PCR for screening selectable marker-free Xa21 transgenic rice<br>利用竞争性PCR筛选无标记Xa21转基因水稻
%A Zhihui Xi
%A Lifen Gao
%A Yuehua Luo
%A Xiaojian Deng
%A Shigui Li
%A Wenxue Zhai
%A <br>夏志辉
%A 高利芬
%A 罗越华
%A 邓晓建
%A 李仕贵
%A 翟文学
%J 生物工程学报
%D 2009
%I 
%X Polymerase chain reaction (PCR) is a simple,quick and highly sensitive method. However the accuracy of the conventional PCR assay was often affected by false positives and false negatives. In this study,a protocol competitive PCR was used to reduce the false results in screening for selectable marker-free (SMF) Xa21 transgenic rice plants. The competitive template of Xa21 was the endogenous Xa21 homologous sequence located on chromosome 11. The competitive template of the selectable marker gene,hygromycin p...
%K competitive PCR
%K false positive
%K false negative
%K selectable marker-free transgenic rice
%K Xa21<br>竞争性PCR
%K 假阳性
%K 假阴性
%K 无标记转基因水稻
%K Xa21
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=35EFB4A27172724E710D675681AF7D07&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=E158A972A605785F&sid=10A39635766FF5D0&eid=44E78A5D1B37D836&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=10