Application of spiral disk column in high-speed counter-current chromatography for peptide and protein separation
螺旋槽圆盘柱高速逆流色谱及其在多肽和蛋白分离中的应用

In order to improve the stationary phase retention of polar solvent systems and aqueous two-phase systems (ATPSs),we designed a multiple spiral disk assembly for type-J high-speed counter-current chromatography (HSCCC). The stationary phase retention was studied under different elution modes by using two solvent systems that contained 1-butanol-acetic acid-water (4:1:5,V/V/V) and polyethylene glycol (PEG) 1000-K2HPO4-water (12.5:12.5:75,W/W/W). The best retention was obtained in L-I-T,U-O-H,L-I-H three mode...