%0 Journal Article
%T Application of spiral disk column in high-speed counter-current chromatography for peptide and protein separation<br>螺旋槽圆盘柱高速逆流色谱及其在多肽和蛋白分离中的应用
%A Guanghui Hu
%A Xueli Cao
%A <br>胡光辉
%A 曹学丽
%J 生物工程学报
%D 2009
%I 
%X In order to improve the stationary phase retention of polar solvent systems and aqueous two-phase systems (ATPSs),we designed a multiple spiral disk assembly for type-J high-speed counter-current chromatography (HSCCC). The stationary phase retention was studied under different elution modes by using two solvent systems that contained 1-butanol-acetic acid-water (4:1:5,V/V/V) and polyethylene glycol (PEG) 1000-K2HPO4-water (12.5:12.5:75,W/W/W). The best retention was obtained in L-I-T,U-O-H,L-I-H three mode...
%K high-speed counter-current chromatography
%K spiral disk assembly
%K peptide
%K protein
%K separation and purification<br>高速逆流色谱
%K 螺旋槽圆盘柱
%K 多肽
%K 蛋白质
%K 分离纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=077B0B06181FB4CEFF5B8020ACDC13DF&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=E158A972A605785F&sid=54E527C5B72E59D8&eid=42FF82ADD37D41AA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=16