Development of SPA-ELISA for detection of antibodies against rabies virus based on expression of main antigenic determinant of Nucleoprotein
狂犬病病毒N蛋白主要抗原区的表达及SPA-ELISA抗体检测方法的建立

To evaluate the effectiveness of rabies vaccination,we developed the SPA-ELISA method to detect the antibodies against rabies virus(RV) using the main antigenic determinant of nucleoprotein(RV N1) as antigen.The complete Nucleoprotein(N) gene and the partial N1 gene(1 000?1 353 bp) of RV Flury LEP strain were amplified using RT-PCR and PCR approaches.The two fragments were inserted into pGEX-6P-1 respectively.Then we transformed the recombinant plasmids into Escherichia coli BL21(DE3) strain and expressed t...