%0 Journal Article
%T Development of SPA-ELISA for detection of antibodies against rabies virus based on expression of main antigenic determinant of Nucleoprotein<br>狂犬病病毒N蛋白主要抗原区的表达及SPA-ELISA抗体检测方法的建立
%A Ni Zeng
%A Miaomiao Gong
%A Liping Guo
%A Wenying Qiu
%A Gang Li
%A <br>曾妮
%A 宫苗苗
%A 郭李平
%A 邱文英
%A 李刚
%J 生物工程学报
%D 2011
%I 
%X To evaluate the effectiveness of rabies vaccination,we developed the SPA-ELISA method to detect the antibodies against rabies virus(RV) using the main antigenic determinant of nucleoprotein(RV N1) as antigen.The complete Nucleoprotein(N) gene and the partial N1 gene(1 000?1 353 bp) of RV Flury LEP strain were amplified using RT-PCR and PCR approaches.The two fragments were inserted into pGEX-6P-1 respectively.Then we transformed the recombinant plasmids into Escherichia coli BL21(DE3) strain and expressed t...
%K rabies virus
%K main antigenic determinant
%K protein A
%K ELISA
%K antibody detection<br>狂犬病病毒，主要抗原区，Proetein
%K A，ELISA，抗体检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=20E65ECF19352841485893E8F43B475E&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=5D311CA918CA9A03&sid=1529CB0C2A6A7E90&eid=CD1AB74EAF0667FF&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=-1&reference_num=12