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生物工程学报 2009
DNA-EGS1386 in cells induced RNase P inhibits the expression of human cytomegalovirus UL49 gene
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Abstract:
External Guide Sequences(EGSs) represents a novel nucleic acid based gene interference approach to modulate gene expression.They are oligonucleotides that consist of a sequence complementary to a target mRNA and recruit intracellular RNase P for specific degradation of the target RNA.DNA-based EGS1386 with a size of 12 nt was chemically synthesized to target the mRNA coding for the UL49 gene of human cytomegalovirus(HCMV).The DNA-based EGS1386 molecule efficiently directed human RNase P to cleave the target...
