High level expression of 5-helix protein in HIV gp41 heptad repeat regions and its virus fusion-inhibiting activity
HIV gp41七肽重复区五螺旋蛋白的高效表达及对病毒融合的抑制

The artificial 5-helix can inhibit the formation of trimer-of-hairpins structure during the course of HIV-directed membrane fusion and then inhibit human immunodeficiency virus (HIV) infecting target cells. But 5-helix was apt to form inclusion body when expressed directly in prokaryotic cell and was difficult to renature, which causes inconvenience to future study. We found a proper expression vector by simulating protein structure. We simulated its proper conformation in two vectors pGEX-6P-1 and pET44b b...