%0 Journal Article
%T High level expression of 5-helix protein in HIV gp41 heptad repeat regions and its virus fusion-inhibiting activity<br>HIV gp41七肽重复区五螺旋蛋白的高效表达及对病毒融合的抑制
%A Jiuqiang Wang
%A Xuwen Pan
%A Po Tien
%A Sidang Liu
%A <br>王久强
%A 潘煦文
%A 田波
%A 刘思当
%J 生物工程学报
%D 2009
%I 
%X The artificial 5-helix can inhibit the formation of trimer-of-hairpins structure during the course of HIV-directed membrane fusion and then inhibit human immunodeficiency virus (HIV) infecting target cells. But 5-helix was apt to form inclusion body when expressed directly in prokaryotic cell and was difficult to renature, which causes inconvenience to future study. We found a proper expression vector by simulating protein structure. We simulated its proper conformation in two vectors pGEX-6P-1 and pET44b b...
%K HIV-1 fusion inhibitors
%K heptad-repeat regions
%K homology modeling<br>HIV-1融合抑制物
%K 七肽重复区
%K 同源建模
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F875CD79CA8A9DF3AD58F952210C4A1F&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=38B194292C032A66&sid=8D75AD3BD0D1BCC5&eid=78976D931AD1540F&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=17