Design and activity analysis of chimeric epidermal growth factor fusion vaccine E5T-mSEA
嵌合表皮生长因子疫苗E5T-mSEA的设计及抑瘤活性检测

Epidermal growth factor receptor (EGFR) and its ligands (EGF and TGFα) are over-expressed in a variety of tumors.Immunization EGF-carrier protein inhibits tumor growth through abrogating binding of EGF to EGFR. Here,a chimeric protein of EGF and TGFα (E5T) was genetically fused to Staphylococcal enterotoxin A (SEA),a bacterial superantigenic protein which promotes humoral B cell response through enhancement of Ag-specific CD4 T cells activity. The resulted fusion proteins were expressed in Escherichia coli and purified though metal chelating affinity chromatography. Immunization of E5T-mSEA fusion protein in mice induced production of high titers antibodies,which recognize both EGF and TGFα. Anti- E5T-mSEA serum at dilution of 1:10 significantly inhibited growth of A431 cell lines but had little effect on 293T cell lines.