%0 Journal Article
%T Design and activity analysis of chimeric epidermal growth factor fusion vaccine E5T-mSEA<br>嵌合表皮生长因子疫苗E5T-mSEA的设计及抑瘤活性检测
%A Qingqing Yin
%A Haiwei Jia
%A Yanhong Zhang
%A Chuanxuan Liu
%A Qingjun Ma
%A Buchang Zhang
%A Hui Zhong
%A Quanbin Xu
%A <br>尹晴晴
%A 贾海威
%A 张艳红
%A 刘传暄
%A 马清钧
%A 张部昌
%A 钟辉
%A 胥全彬
%J 生物工程学报
%D 2010
%I 
%X Epidermal growth factor receptor (EGFR) and its ligands (EGF and TGFα) are over-expressed in a variety of tumors.Immunization EGF-carrier protein inhibits tumor growth through abrogating binding of EGF to EGFR. Here,a chimeric protein of EGF and TGFα (E5T) was genetically fused to Staphylococcal enterotoxin A (SEA),a bacterial superantigenic protein which promotes humoral B cell response through enhancement of Ag-specific CD4 T cells activity. The resulted fusion proteins were expressed in Escherichia coli and purified though metal chelating affinity chromatography. Immunization of E5T-mSEA fusion protein in mice induced production of high titers antibodies,which recognize both EGF and TGFα. Anti- E5T-mSEA serum at dilution of 1:10 significantly inhibited growth of A431 cell lines but had little effect on 293T cell lines.
%K EGFR
%K mSEA
%K E5T
%K tumor vaccine<br>表皮生长因子，mSEA，E5T，肿瘤疫苗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=8D4CA026CAE2268920B66999A4F5E22E&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=38B194292C032A66&sid=FA88DCCE84EA0A56&eid=FA3423FC1AE95C4E&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=6