Establishment of xenograft mouse models to study human lung cancer by using in vivo imaging system
用于活体成像的小鼠肺癌移植瘤模型的建立

We established xenograft mouse models for studying human lung cancer by using an in vivo imaging system.We first transfected pGL4.17(luc2/neo) plasmid into human non-small lung cancer A549 cells and screened cell lines stably expressing a luciferase reporter gene with G418.Then we analyzed the correlation of luciferase activity and cells number by in vitro bioluminescence.Furthermore,we compared cell growth characteristics by cell counting.We selected suitable clones and inoculated subcutaneously into nude ...