PURIFICATION OF SERUM IGM FROM GRASS CARP(CIENOYHARYNGODONI DELLUS) AND PREPARATION OF RABBIT SERA ANTI-IGM
草鱼血清IgM蛋白的纯化及抗血清的制备

Immunoglobulins are the primary humoral component of the acquired immune system.In order to know the ImmunoglobulinM(IgM) of grass carp(Cienoyharyngodoni dellus),two different isolating methods were applied to find the best way to purify serum IgM in the present study.The purity and molecular weight of the serum IgM were determined,and then the rabbit polyclonal antisera were prepared with the purified IgM.In this study,blood was collected with a syringe from the caudal sinus of 30 fish,and allowed to clot ...