%0 Journal Article
%T PURIFICATION OF SERUM IGM FROM GRASS CARP(CIENOYHARYNGODONI DELLUS) AND PREPARATION OF RABBIT SERA ANTI-IGM<br>草鱼血清IgM蛋白的纯化及抗血清的制备
%A DING Wei-Dong
%A CAO Li-Ping
%A CAO Zhe-Ming
%A <br>丁炜东
%A 曹丽萍
%A 曹哲明
%J 水生生物学报
%D 2010
%I 
%X Immunoglobulins are the primary humoral component of the acquired immune system.In order to know the ImmunoglobulinM(IgM) of grass carp(Cienoyharyngodoni dellus),two different isolating methods were applied to find the best way to purify serum IgM in the present study.The purity and molecular weight of the serum IgM were determined,and then the rabbit polyclonal antisera were prepared with the purified IgM.In this study,blood was collected with a syringe from the caudal sinus of 30 fish,and allowed to clot ...
%K Grass crap
%K Protein A-sepharose affinity chromatography
%K Immunoglobulin M(IgM)
%K Rabbit sera anti-IgM
%K ELISA<br>草鱼
%K 蛋白A亲和层析法
%K 免疫球蛋白
%K 兔抗鱼IgM血清
%K 酶联免疫吸附分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=A345869C012EA4960D3F15448E10DCF5&yid=140ECF96957D60B2&vid=339D79302DF62549&iid=CA4FD0336C81A37A&sid=F260CE035846B3B8&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=0&reference_num=31