Construction and fermentation of a recombinant Candida glycerinogenes strain with high glycerol production
新一代高产甘油重组菌的构建及其初步发酵

Candida glycerinogenes WL2002-5(C.g) is an important industrial strain for glycerol production.To further improve glycerol production,we reconstructed a binary vector pCAM3300-zeocin-CgGPD1,introduced it to Agrobacterium tumefaciens LBA4404 by electroporation,and then transformed the T-DNA harboring the CgGPD1 to Candida glycerinogenes by Agrobacterium tumefaciens-mediated transformation(ATMT).After 96 h fermentation with glucose as the substrate,we screened a transformant named C.g-G8 with high glycerol pr...