%0 Journal Article
%T Construction and fermentation of a recombinant Candida glycerinogenes strain with high glycerol production<br>新一代高产甘油重组菌的构建及其初步发酵
%A Ailing Liu
%A Zhiming Rao
%A Zheng M
%A Bin Zhuge
%A Huiying Fang
%A Jian Zhuge
%A <br>刘爱玲
%A 饶志明
%A 马正
%A 诸葛斌
%A 方慧英
%A 诸葛健
%J 生物工程学报
%D 2009
%I 
%X Candida glycerinogenes WL2002-5(C.g) is an important industrial strain for glycerol production.To further improve glycerol production,we reconstructed a binary vector pCAM3300-zeocin-CgGPD1,introduced it to Agrobacterium tumefaciens LBA4404 by electroporation,and then transformed the T-DNA harboring the CgGPD1 to Candida glycerinogenes by Agrobacterium tumefaciens-mediated transformation(ATMT).After 96 h fermentation with glucose as the substrate,we screened a transformant named C.g-G8 with high glycerol pr...
%K Candida glycerinogenes
%K glycerol-3-phosphate dehydrogenase
%K Agrobacterium tumefaciens-mediated transformation<br>产甘油假丝酵母
%K 胞浆-3-磷酸甘油脱氢酶
%K 根癌农杆菌介导转化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=7E429675084B3EF6DA3DCA31A56FBFAA&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=7C8C2BAFC9BA0571&eid=793F041A4288469A&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=20