Construction and expression of the fusion gene CCK39/UreB in recombinant Escherichial coli BL-21(DE3)
CCK39/UreB双基因融合表达载体的构建及其在大肠杆菌中的表达

The aims of this research were to construct prokaryotic expression vector containing fusion gene of Cholecystokinin 39(CCK39) of pig and Urease subunit B(UreB) of coliform bacteria,and then to express the fusion protein in reconbinant Escherichia coli BL21(DE3).The CCK39 gene was amplified by RT-PCR from the extracted total RNA of pig's duodenum,and the UreB gene was then amplified by PCR from the extracted plasmid DNA of bacillus of coliform bacteria from pig's intestinal content.Then the CCK39 and the Ure...