%0 Journal Article
%T Construction and expression of the fusion gene CCK39/UreB in recombinant Escherichial coli BL-21(DE3)<br>CCK39/UreB双基因融合表达载体的构建及其在大肠杆菌中的表达
%A Weiyang Zhang
%A Yan Li
%A Tongshan Wu
%A Wenhua Luo
%A Bin Hu
%A Wenfeng Hu
%A <br>张炜阳
%A 李岩
%A 吴同山
%A 罗文华
%A 胡斌
%A 胡文锋
%J 生物工程学报
%D 2009
%I 
%X The aims of this research were to construct prokaryotic expression vector containing fusion gene of Cholecystokinin 39(CCK39) of pig and Urease subunit B(UreB) of coliform bacteria,and then to express the fusion protein in reconbinant Escherichia coli BL21(DE3).The CCK39 gene was amplified by RT-PCR from the extracted total RNA of pig's duodenum,and the UreB gene was then amplified by PCR from the extracted plasmid DNA of bacillus of coliform bacteria from pig's intestinal content.Then the CCK39 and the Ure...
%K CCK39/UreB<br>融合表达
%K 脲酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=53F79E0C8D90F8E7C1D358955CE6217B&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=C5DDA626F7B5506E&eid=8595ABBDD5FF38F7&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=16