Anti-angiogenic activities of Lj-RGD3 toxin protein from Lampetra japonica and its mutation protein Lj-112 without RGD motifs
日本七鳃鳗Arg-Gly-Asp毒素蛋白Lj-RGD3野生型与RGD全缺失突变体Lj-112的抗血管新生作用

Arg-Gly-Asp(RGD)-toxin protein Lj-RGD3 of Lampetra japonica shares homologous with a Histidine-rich glycoprotein(HRG),and both RGD-toxin protein and HRG have antiangiogenic activities with different targets.To study the relationship between the function and the structure of Lj-RGD3,we studied the anti-angiogenic characteristics of both Lj-RGD3 and the mutation named Lj-112 of which three RGD motifs of Lj-RGD3 were deleted.We synthesized the gene of Lj-112,constructed it to the plasmid pET23b,and expressed t...